Keith J. Stine

Professor Stine received his BS from Fairleigh Dickinson University and his Ph.D. from MIT. He was a postdoctoral fellow at UCLA and joined the UMSL faculty in 1990. He served as Chair of the Faculty Senate and University Assembly for two years and was appointed department chair on Aug. 1 2019.

kstine@umsl.edu
Office: M204
Chair Office B316A
Phone: (314) 516-5346
Fax: (314) 516-5342

Research Interests

Dr. Stine's research effort involves studies of modified surfaces and nanostructures. The surface modification of nanostructures is pursued with a focus on their prospective applications in bioanalytical chemistry such as in immunoassays, sensors, or in separations. Immobilization of proteins onto nanostructures of gold and other materials is pursued by adsorption or by covalent linkage to self-assembled monolayers. The characterization of these nanostructures by microscopy (SEM, TEM, AFM) is actively pursued. The bioanalytical application of these materials is pursued using primarily electrochemical methods such as impedance spectroscopy and cyclic voltammetry. Nanoporous gold and other related materials are of particular interest due to their high surface area and capacity to be surface modified, as well as their support of localized surface plasmon resonance (LSPR). The preparation and characterization of these materials uses a range of electrochemical techniques and other analytical methods including gas adsorption isotherm analysis for determination of pore size, distribution, and surface area, and thermogravimetric analysis (TGA) for analysis of surface loading. Other projects concern the study of lipid monolayers and bilayers as models of processes occurring at the surface of cell membranes, and the use of these monolayers in molecular recognition studies. Monolayers are studied using surface pressure isotherms and Brewster angle microscopy. A special interest is in the field of carbohydrate - protein interactions at interfaces, motivated by the broad biological significance of carbohydrates. The field of supported synthesis of carbohydrates using nanoporous gold and related materials is also actively pursued in collaboration with the Demchenko lab.

Selected Publications

"Methods to Generate Structurally Hierarchical Architectures in Nanoporous Coinage Metals", P. Sondhi and K. J. Stine, Coatings, 2021, 11, 1440.

“Electrochemical Sandwich Assays for Biomarkers Incorporating Aptamers, Antibodies and Nanomaterials for Detection of Specific Protein Biomarkers.”, D. Neupane and K. J. Stine, Applied Sciences, 2021, 11, 7087.

“HPLC-Based Automated Oligosaccharide Synthesis”, M. Shadrick, M. Panza, V. Ganesh, S. G. Pistorio, K. J. Stine, and A. V. Demchenko in Comprehensive Glycoscience, 2nd Edition, volume 2 (S. Vidal, Editor), Elsevier, 2021, 623-636.

“Nanostructured Materials for Glycan based Applications”, K. J. Stine, J. K. Bhattarai, Md H. Uddin Maruf, D. Neupane, B. Nepal, and P. Sondhi in Comprehensive Glycoscience, 2nd Edition, volume 4 (R. Narain,  Editor), Elsevier, 2021, 474-504.

″Nanoporous Gold Monolith for High Loading of Unmodified Doxorubicin and Sustained Co-Release of Doxorubicin-Rapamycin″, J. K Bhattarai, D. Neupune, B. Nepal, A. V. Demchenko and K. J. Stine,  Nanomaterials 2021, 11, 208.

“A Streamlined Regenerative Glycosylation Reaction: Direct, Acid-Free Activation of Thioglycosides S. Escopy, Y. Singh, K. J. Stine, and A. V. Demchenko. Chem. Eur. J. 2021, 27, 354.

″Structure,Formation, and Biological Interactions of Supported Lipid Bilayers (SLB) Incorporating Lipopolysaccharide″, P. Sondhi, D. Lingden, and K. J. Stine, Coatings 2020, 10, 981.

″The development of a dedicated polymer support for the solid phase oligosaccharide synthesis″, M. Panza, D. Neupane, K. J. Stine and A. V. Demchenko. Chem. Comm., 2020, 56, 10568.

″Adhesion layer-free attachment of gold on silicon wafer and its application in localized surface plasmon resonance-based biosensing″, J. K. Bhattarai, D. Neupane, B. Nepal, M. D. Alharthi, A. V. Demchenko and K. J. Stine, Sensors and Actuators A, 2020, 312, 112155.

″A pH sensitive thiolated β-cyclodextrin-modified nanoporous gold for controlled release of doxorubicin", D. Neupane, J. K. Bhattarai, A. V. Demchenko and K. J. Stine, J. Drug. Deliv. Sci. Technol. 2020, 101985.

″Nanomaterials for Biosensing Lipopolysaccharide″, P. Sondhi, M. H. U. Maruf and K. J. Stine, Biosensors 2020, 10, 2.

″HPLC-assisted automated oligosaccharide synthesis: the implementation of the two-​way split valve as a mode of complete automation,″ M. Panza, K. J. Stine and A. V. Demchenko, Chem. Comm.. 2020, 56, 1333.

″Plasmonic-Active Nanostructured Thin Films,″  J. K. Bhattarai, M. H. U. Maruf and K. J. Stine, Processes 2020, 8, 115.

″Chemical synthesis of human milk oligosaccharides: lacto-N-neohexaose (Galβ1 → 4GlcNAcβ1→)2 3,6Galβ1 → 4Glc,″ M. D. Bandara, K. J. Stine, and A. V. Demchenko, Org. & Biomol. Chem. 2020, 18, 1747

″Glycoalkaloids: structure, properties, and interactions with model membrane systems,″ B. Nepal and K. J. Stine, Processes, 2019, 7, 513

″The chemical synthesis of human milk oligosaccharides: Lacto-N-tetraose (Galβ1→3GlcNAcβ1→3Galβ1→4Glc),″ M. D. Bandara, K. J. Stine, and A. V. Demchenko, Carbohydrate Res. 2019, 486, 107824.

″Chemical Synthesis of Human Milk Oligosaccharides: Lacto-N-hexaose Galβ1→3GlcNAcβ1→3 [Galβ1→4GlcNAcβ1→6] Galβ1→4Glc,″ M. D. Bandara, K. J. Stine, and A. V. Demchenko, J. Org. Chem. 2019, 84, 16192

″Nanoporous gold and other related materials,″,K. J. Stine, J. Nanomaterials, 2019, 9, 1081

″Biosensor applications of electrodeposited nanostructures,″ K. J. Stine, J. Appl. Sciences, 2019, 9, 797.

“Monolayers of Carbohydrate-Containing Lipids at the Water- Air Interface,“ B. Nepal and K. J. Stine,  In "Cell Culture,” Ed. R. A. Mehanna, Ch. 11, Intechopen, 2019.

Complete list of publications