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Keith J. Stine

Keith J. Stine

Professor Stine received his BS from Fairleigh Dickinson University and his Ph.D. from MIT. He was a postdoctoral fellow at UCLA and joined the UMSL faculty in 1990. He served as Chair of the Faculty Senate and University Assembly for two years and was appointed department chair on Aug. 1 2019.

Office: N204
Phone: (314) 516-5346
Fax: (314) 516-5342

Research Interests

Dr. Stine's research effort involves studies of modified surfaces and nanostructures. The surface modification of nanostructures is pursued with a focus on their prospective applications in bioanalytical chemistry such as in immunoassays, sensors, or in separations. Immobilization of proteins onto nanostructures of gold and other materials is pursued by adsorption or by covalent linkage to self-assembled monolayers. The characterization of these nanostructures by microscopy (SEM, TEM, AFM) is actively pursued. The bioanalytical application of these materials is pursued using primarily electrochemical methods such as impedance spectroscopy and cyclic voltammetry. Nanoporous gold and other related materials are of particular interest due to their high surface area and capacity to be surface modified, as well as their support of localized surface plasmon resonance (LSPR). The preparation and characterization of these materials uses a range of electrochemical techniques and other analytical methods including gas adsorption isotherm analysis for determination of pore size, distribution, and surface area, and thermogravimetric analysis (TGA) for analysis of surface loading. Other projects concern the study of lipid monolayers and bilayers as models of processes occurring at the surface of cell membranes, and the use of these monolayers in molecular recognition studies. Monolayers are studied using surface pressure isotherms and Brewster angle microscopy. A special interest is in the field of carbohydrate - protein interactions at interfaces, motivated by the broad biological significance of carbohydrates. The field of supported synthesis of carbohydrates using nanoporous gold and related materials is also actively pursued in collaboration with the Demchenko lab.

Selected Publications

″Manual and Automated Syntheses of the N-Linked Glycoprotein Core Glycans,″ S. G. Pistorio, S. Geringer, K. J. Stine and A. V. Demchenko, J. Org. Chem. 2019, 84, 6576.

″The chemical synthesis of human milk oligosaccharides: Lacto-N-neotetraose (Galβ1→4GlcNAcβ1→3Galβ1→4Glc),″ M. D. Bandara, K. J. Stine and A. V. Demchenko, Carbohydr. Res. 2019, 483, 107743.

“Monolayers of Carbohydrate-Containing Lipids at the Water- Air Interface,“ B. Nepal and K. J. Stine,  In "Cell Culture”, Ed. R. A. Mehanna, Ch. 11, Intechopen, 2019.

″Investigation of Glycosyl Nitrates as Building Blocks for Chemical Glycosylation,″ T.Wang, Y. Singh, K. J.  Stine and A. V. Demchenko, Eur. J. Org. Chem. 2018, 47, 6699.

″Automated Chemical Oligosaccharide Synthesis: Novel Approach to Traditional Challenges,″ M. Panza, S. G. Pistorio, K. J. Stine and A. V. Demchenko,  Chem. Rev. 2018, 118, 8105.

″Immobilization of glycans on solid surfaces for application in glycomics, ″ C. L. O'Neil, K. J  Stine and A. V. Demchenko, J. Carbohydrate Chem. 2018, 37, 225.

″Glycosyl nitrates in synthesis: streamlined access to glucopyranose building blocks differentiated at C-2,″ T. Wang, S. S. J. P. Yasomanee, N. P. Rath, Nigam K. J. Stine and A. V. Demchenko, Org. & Biomol. Chem. 2018, 16, 3596.

″Nanoporous Metals by Alloy Corrosion: Bioanalytical and Biomedical Applications″, E. Seker, W.-C. Shih, and K. J. Stine, Materials Research Society Bulletin, 2018, 43, 45.

″Preparation, modification, characterization, and biosensing application of nanoporous gold using electrochemical techniques″, J. K. Bhattarai, D. Neupane, B. Nepal, V. Mikhaylov, A. V. Demchenko and K. J. Stine, Nanomaterials 2018, 8, 171/1.

″Regenerative Glycosylation,″ Y. Singh, T. Wang, S. A. Geringer, K. J. Stine and A. V. Demchenko, J. Org. Chem. 2018, 83, 374.

″Nanoporous gold for enzyme immobilization″, K. J. Stine, K.  Jefferson and O. Shulga, Methods in Molecular Biology 2017, 1504 (Enzyme Stabilization and Immobilization), pp 37-60.

″Application of Porous Materials to Carbohydrate Chemistry and Glycoscience″, K. J. Stine, Advances in Carbohydrate Chemistry and Biochemistry, 2017, 74, 61.

“Enzyme Immobilization on Nanoporous Gold – A Review”, K. J Stine, Biochemistry Insights, 2017, 10, 1.

″Self-assembled Monolayers of Carbohydrate Derivatives on Gold Surfaces″, J. K. Bhattarai, D. Neupane, V. Mikhaylov, A. V. Demchenko, and K. J. Stine, Carbohydrates, Intech Publishers, 2017, edited by M. Caliskan, I. H. Kavakli and G. C. Oz, pp. 63-97.

″Localized surface plasmon resonance active surfaces applied to study carbohydrate–protein and protein–protein interactions″, J. K. Bhattarai, D. Neupane, V.Mikhaylov, A. V. Demchenko, and K. J. Stine, Nova Science Publishers, Surface Plasmon Resonance (SPR): Advances in Research and Applications, (Ed. D. Howell), 2017, pp. 87-122.

Complete list of publications