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Keith J. Stine

Keith J. Stine

Professor Stine graduated with special honors with a B.S. in Chemistry from Fairleigh Dickinson University and received his Ph.D. from Massachusetts Institute of Technology. He was a postdoctoral fellow at UCLA and joined the UMSL Chemistry Department in the Fall of 1990 and served as the Chair of the Faculty Senate and University Assembly recently for two years..

kstine@umsl.edu
Office: N204
Phone: (314) 516-5346
Fax: (314) 516-5342

Research Interests

Dr. Stine's research effort involves studies of modified surfaces and nanostructures. The surface modification of nanostructures is pursued with a focus on their prospective applications in bioanalytical chemistry such as in immunoassays, sensors, or in separations. Immobilization of proteins onto nanostructures of gold and other materials is pursued by adsorption or by covalent linkage to self-assembled monolayers. The characterization of these nanostructures by microscopy (SEM, TEM, AFM) is actively pursued. The bioanalytical application of these materials is pursued using primarily electrochemical methods such as impedance spectroscopy and cyclic voltammetry. Nanoporous gold and other related materials are of particular interest due to their high surface area and capacity to be surface modified, as well as their support of localized surface plasmon resonance (LSPR). The preparation and characterization of these materials uses a range of electrochemical techniques and other analytical methods including gas adsorption isotherm analysis for determination of pore size, distribution, and surface area, and thermogravimetric analysis (TGA) for analysis of surface loading. Other projects concern the study of lipid monolayers and bilayers as models of processes occurring at the surface of cell membranes, and the use of these monolayers in molecular recognition studies. Monolayers are studied using surface pressure isotherms and Brewster angle microscopy. A special interest is in the field of carbohydrate - protein interactions at interfaces, motivated by the broad biological significance of carbohydrates. The field of supported synthesis of carbohydrates using nanoporous gold and related materials is also actively pursued in collaboration with the Demchenko lab.

Selected Publications

″Automated Chemical Oligosaccharide Synthesis: Novel Approach to Traditional Challenges,″ M. Panza, S. G. Pistorio, K. J. Stine and A. V. Demchenko,  Chem. Rev. 2018, Ahead of Print

″Immobilization of glycans on solid surfaces for application in glycomics, ″ C. L. O'Neil, K. J  Stine and A. V. Demchenko, J. Carbohydrate Chem. 2018, Ahead of Print

″Glycosyl nitrates in synthesis: streamlined access to glucopyranose building blocks differentiated at C-2,″ T. Wang, S. S. J. P. Yasomanee, N. P. Rath, Nigam K. J. Stine and A. V. Demchenko, Org. & Biomol. Chem. 2018, 16, 3596

″Nanoporous Metals by Alloy Corrosion: Bioanalytical and Biomedical Applications″, E. Seker, W.-C. Shih, and K. J. Stine, Materials Research Society Bulletin, 2018, 43, 45.

″Preparation, modification, characterization, and biosensing application of nanoporous gold using electrochemical techniques″, J. K. Bhattarai, D. Neupane, B. Nepal, V. Mikhaylov, A. V. Demchenko and K. J. Stine, Nanomaterials 2018, 8, 171/1

″Nanoporous gold for enzyme immobilization″, K. J. Stine, K.  Jefferson and O. Shulga, Methods in Molecular Biology 2017, 1504 (Enzyme Stabilization and Immobilization),pp 37-60

″Application of Porous Materials to Carbohydrate Chemistry and Glycoscience″, K. J. Stine, Advances in Carbohydrate Chemistry and Biochemistry, 2017, 74, 61

″Regenerative Glycosylation,″ Y. Singh, T. Wang, S. A. Geringer, K. J. Stine and A. V. Demchenko, J. Org. Chem. 2018, 83, 374.

“Enzyme Immobilization on Nanoporous Gold – A Review”, K. J Stine, Biochemistry Insights, 2017, 10, 1.

″Self-assembled Monolayers of Carbohydrate Derivatives on Gold Surfaces″, J. K. Bhattarai, D. Neupane, V. Mikhaylov, A. V. Demchenko, and K. J. Stine, Carbohydrates, Intech Publishers, 2017, edited by M. Caliskan, I. H. Kavakli and G. C. Oz, pp. 63-97.

″Localized surface plasmon resonance active surfaces applied to study carbohydrate–protein and protein–protein interactions″, J. K. Bhattarai, D. Neupane, V.Mikhaylov, A. V. Demchenko, and K. J. Stine, Nova Science Publishers, Surface Plasmon Resonance (SPR): Advances in Research and Applications, (Ed. D. Howell), 2017, pp. 87-122.

″Carbohydrate-Protein Interactions Studied Using Electrochemical Impedance Spectroscopy″, J. K. Bhattarai, V. Mikhaylov, D. Neupane, B. Nepal, A. V. Demchenko, and K. J. Stine, Nova Science Publishers, Electrochemical Impedance Spectroscopy: Methods, Analysis and Research, (Ed. J. Brock), 2017, pp. 1-28

″OFox imidates as versatile glycosyl donors for chemical glycosylation,″ S. S. Nigudkar, T. Wang, S. G. Pistorio, J. P. Yasomanee, K. J. Stine and A. V. Demchenko, Org. & Biomol. Chem. 2017, 15, 348.

″HPLC-Assisted Automated Oligosaccharide Synthesis: Implementation of the Autosampler as a Mode of the Reagent Delivery,″ S. G. Pistorio, S. S. Nigudkar, K. J. Stine and A. V. Demchenko, J. Org. Chem. 2016, 81, 8796

"Electrochemical impedance spectroscopy study of carbohydrate-terminated alkanethiol monolayers on nanoporous gold: Implications for pore wetting,″ A. Sharma, J. K. Bhattarai, S. S. Nigudkar, S. G. Pistorio, A. V. Demchenko and K. J. Stine, J. Electroanal. Chem. 2016, 782, 174.

 

Complete list of publications