Keith J. Stine

Professor Stine graduated with special honors with a B.S. in Chemistry from Fairleigh Dickinson University and received his Ph.D. from Massachusetts Institute of Technology. He was a postdoctoral fellow at UCLA and joined the UM-St. Louis Chemistry Department in the Fall of 1990.

Research Interests

Dr. Stine's research effort involves studies of modified surfaces and nanostructures. The surface modification of nanostructures is pursued with a focus on their prospective applications in bioanalytical chemistry such as in immunoassays, sensors, or in separations. Immobilization of proteins onto nanostructures of gold and other materials is pursued by adsorption or by covalent linkage to self-assembled monolayers. The characterization of these nanostructures by microscopy (SEM, TEM, AFM) is actively pursued. The bioanalytical application of these materials is pursued using primarily electrochemical methods such as impedance spectroscopy and cyclic voltammetry. Nanoporous gold and other related materials are of particular interest due to their high surface area and capacity to be surface modified, as well as their support of localized surface plasmon resonance (LSPR). The preparation and characterization of these materials uses a range of electrochemical techniques and other analytical methods including gas adsorption isotherm analysis for determination of pore size, distribution, and surface area, and thermogravimetric analysis (TGA) for analysis of surface loading. Other projects concern the study of lipid monolayers and bilayers as models of processes occurring at the surface of cell membranes, and the use of these monolayers in molecular recognition studies. Monolayers are studied using surface pressure isotherms and Brewster angle microscopy. A special interest is in the field of carbohydrate - protein interactions at interfaces, motivated by the broad biological significance of carbohydrates. The field of supported synthesis of carbohydrates using nanoporous gold and related materials is also actively pursued in collaboration with the Demchenko lab.

Selected Publications

“Regenerative Glycosylation under Nucleophilic Catalysis”, S. S. Nigudkar, K. J. Stine and  A. V. Demchenko, J. Am. Chem. Soc., 2014, 126, 921.

"Square-wave voltammetry assays for glycoproteins on nanoporous gold," B. Pandey, J. K. Bhattarai, P. Pornsuriyasak, K. Fujikawa,R. Catania, A. V. Demchenko and K. J. Stine, J. Electroanal. Chem. 2014, 717/718, 47.

"The influence of gold surface texture on microglia morphology and activation,"  Y. H. Tan, S. Terrill, G. S. Paranjape, K. J. Stine and M. R. Nichols, Biomat. Sci. 2014, 2, 110.

"Surface-Tethered Iterative Carbohydrate Synthesis: A Spacer Study," V. N. Ganesh, K. Fujikawa, Y-H. Tan, S. S. Nigudkar, K. J. Stine and A. V. Demchenko,  J. Org. Chem. 2013, 78, 6849. 

"O-Benzoxazolyl imidates as versatile glycosyl donors for chemical glycosylation," S. S. Nigudkar, A. R. Parameswar, P. Pornsuriyasak, K. J. Stine, and A. V. Demchenko, Org. and Biomol. Chem 2013, 11 4068.

"Lectin-Carbohydrate Interactions on Nanoporous Gold Monoliths," Y.-H. Tan, K. Fujikawa, P. Pornsuriyasak, A. J. Alla, A. V. Demchenko and K. J. Stine  New J. Chem., 2013, 37, 2150

“Electrochemical Characterization of Globotriose-Containing Self-Assembled Monolayers on Nanoporous Gold and their Binding of Soybean Agglutinin”, B. Pandey, Y. H. Tan,  A. R. Parameswar, P. Pornsuriyasak, A. V. Demchenko and K.  J. Stine, Carbohydrate Research, 2012, 373, 9

"Nanoporous gold as a solid support for protein immobilization and development of an electrochemical immunoassay for prostate specific antigen and carcinoembryonic antigen", B. Pandey, A. V. Demchenko and K. J. Stine, Microchimica Acta, 2012, 179, 71.

“Comparative Study of the Binding of Concanavalin A to Self-Assembled Monolayers Containing a Thiolated α-Mannoside on Flat Gold and on Nanoporous Gold,” B. P. Pandey, Y. H. Tan, K. Fujikawa, A. V.Demchenko and K. J. Stine, J.  Carbohydrate Chem. 2012, 31, 466.

"HPLC-Assisted Automated Oligosaccharide Synthesis," N. Vijaya Ganesh, Kohki Fujikawa, Yih Horng Tan, Keith J. Stine and Alexei V. Demchenko, Org. Lett. 2012, 14, 3036

“Surface Area and Pore Size Characteristics of Nanoporous Gold Subjected to Thermal, Mechanical, or Chemical Modifications studied using Gas Adsorption Isotherms, Cyclic Voltammetry, and Scanning Electron Microscopy,” Y-H. Tan, J. A. Davis, K. Fujikawa, N. V. Ganesh, A. V. Demchenko and K. J. Stine, J. Mat. Chem., 2012, 22, 6733.

"Bioconjugation Reactions for Covalent Coupling of Proteins to Gold Surfaces", Y. H. Tan, B. Pandey, A. Sharma, J. Bhattarai, and K. J. Stine, Global J. Biochem. 20123, 6.

"Brewster Angle Microscopy", K. J. Stine, in Supramolecular Chemistry: From Molecules to Nanomaterials, J. W. Steed and P. A. Gale (eds).  John Wiley & Sons Ltd, Chichester, UK, 2012, pp. 589-618.

 "Reverse Orthogonal Strategy for Oligosaccharide Synthesis", K. Fujikawa, N. V. Ganesh, Y. H. Tan, K. J Stine and A. V. Demchenko, Chem. Commun2011, 10602

"Atomic Force Microscopy Characterization of Lipid/Protein Nanostructures Formed in Langmuir-Blodgett Films", Y. H. Tan and K. J. Stine, in Langmuir Monolayers in Thin Film Technology edited by J. A. Sherwin, Nova Science Publishers (Hauppage, New York), 2011 pp. 101-130,.

Complete list of publications