Cynthia M. Dupureur
Professor Dupureur received her B.S. degree from Southwest Missouri State University, and her Ph.D. from Ohio State University. She joined the UM-St. Louis Chemistry faculty in 2001. She held a faculty position at Texas A&M following postdoctoral fellowship at the California Institute of Technology.
Phone: (314) 516-4392
Fax: (314) 516-5342
My group is interested in structure-function relationships for biomolecules. This involves developing an understanding of how structural elements give rise to specific functions like enzymatic catalysis and molecular recognition. For a number of years, my group has explored restriction enzymes and how they combine DNA sequence recognition with metal ion-mediated hydrolytic chemistry. More recently we have turned our hands to collaborative projects with synthetic chemists. One involves the study of esterases, with a particular interest in the diabetes drug target hormone sensitive lipase. A newer project involves biophysical studies of DNA interactions with polyamides, which show promise as HPV drugs. Over the history of the group, our studies have involved a wide array of techniques including basic molecular biology, enzyme kinetics, computational chemistry, calorimetry, and fluorescence and NMR spectroscopies. This research style provides excellent opportunities to master a number of biophysical and mechanistic approaches.
"Fluorescence assay of polyamide-DNA interactions," C. M. Dupureur, J. K. Bashkin, K. Aston, K. J. Koeller, K. R. Gaston and G. He, Analytical Biochem. 2012, 423, 178.
"DNA targeting and cleavage by an engineered metalloprotein dimer," S. W. Wong-Deyrup, C. Prasannan, C. M. Dupureur and S. J. Franklin, J. Biol. Inorg. Chem. 2012, 17, 387.
"Metal ion and DNA binding by single-chain PvuII endonuclease: lessons from the linker," G. A. Papadakos and C. M. Dupureur, J. Biol. Inorg. Chem. 2011, 16, 1269.
"The First Total Synthesis of ()-Cyclophostin and ()-Cyclipostin P: Inhibitors of the Serine Hydrolases Acetyl Cholinesterase and Hormone Sensitive Lipase", R. K. Malla, S Bandyopadhyay, C. D. Spilling, S. Dutta and C. M. Dupureur, Organic Lett. 2011, 13, 3094.
"One is enough: insights into the two-metal ion nuclease mechanism from global analysis and computational studies", C. M. Dupureur, Metallomics, 2010, 2, 609.
"Nucleophile activation in PD...(D/E)xK metallonucleases: An experimental and computational pKa study", F. Xie, J. M. Briggs and C. M. Dupureur, J. Inorg. Biochem. 2010, 104, 665.
"Synthesis and kinetic analysis of some phosphonate analogs of cyclophostin as inhibitors of human acetylcholinesterase". S. Dutta, R. K. Malla, S. Bandyopadhyay, C. D. Spilling, and C. M. Dupureur, Bioorg. & Med. Chem. 2010, 18, 2265.
"Characterizing metalloendonuclease mixed metal complexes by global kinetic analysis", C. B. Prasannan, F. Xie and C. M. Dupureur, J. Biol. Inorg. Chem 2010 15, 533.
"Kinetic analysis of product release and metal ions in a metallonuclease" F. Xie and C. M. Dupureur, Arch. Biochem. Biophys. 2009, 483, 1.
"One- and Two-Metal Ion Catalysis: Global Single-Turnover Kinetic Analysis of the PvuII Endonuclease Mechanism" F. Xie, S-H. Qureshi, G. Papadakos and C. M. Dupureur, Biochemistry 2008, 47, 12540
"Synthesis and Biological Evaluation of a Phosphonate Analog of the Natural Acetyl Cholinesterase Inhibitor Cyclophostin" S. Bandyopadhyay, S. Dutta, C. D. Spilling, C. M. Dupureur and N. P. Rath, J. Org. Chem. 2008, 73, 8386
"An integrated look at metallonuclease mechanism". C. M. Dupureur, Curr Chem Biol 2008, 12, 250
"Roles of metal ions in nucleases". C. M. Dupureur, Curr Opin Chem Biol 2008, 12, 1
"Uncoupling Metallonuclease Metal ion Binding Sites via Nudge Mutagenesis", G. A. Papadakos, H. Nastri, P. Riggs and C. M. Dupureur, J. Biol. Inorg. Chem. 2007, 12, 557
"Unique, 31P spectral response to the formation of a specific restriction enzyme-DNA complex", C. M. Dupureur, Nucleosides, Nucleotides & Nucleic Acids, 2006, 25, 747