Center for Nanoscience

Mass Spectrometry Facility

 

The mass spectrometry facility is housed in a 1000 sq ft laboratory located in the UMSL Research Building (R003).  In addition to the three mass spectrometers described below, there are areas for data processing, instrument maintenance, parts storage and sample preparation.  The instrumentation is used primarily for support of research and teaching in teh Department of Chemistry and Biochemistry, however in years past the MS facility has been a resource for local businesses and members of the academic community that lack this instrumentation.

Hewlett Packard GC/MS System Model 5988A

For routine mass spectral analysis following capillary column gas chromatographic (GC) separation with:

  1. Electron impact (EI) and chemical ionization (CI) capabilities
  2. Positive and negative ion detection
  3. An extended-mass quadrupole
  4. A solids probe

The HP 5988 GC-MS is equipped with a recently purchased PC-based version of HP's Chem Station data system which interfaces directly with the NIST MS Data Base; independent data processing can also be accomplished using the Automated Mass Spectral Deconvolution and Identification System (AMDIS) developed at NIST.  A user-friendly instrument, the HP 5988 is primarily in electron impact (EI) mode and for compunds having mass less than ca 400 Da.

JEOL MStation [JMS-700] Mass Spectrometer

A high-performance magnetic sector mass spectrometer for both high and low resolution mass spectral analysis equipped for:

  1. Fast Atom Bombardment (FAB) ionization, Electrospray Ionization (ESI) and Atmospheric Ion Chemical Ionization (APCI) as EI and CI methods
  2. Positive and negative on detection
  3. Linked scan measurements

A combination source operating in either chemical ionization (CI), fast atom bombardment (FAB) or electron impact (EI) mode is most commonly employed.  Double focusing capability provides accurate mass (+/- 1 mmu) if so desired; with appropriate calibration compounds, mass determination to several thousand Da can be routine.  Mass spectra of literally hundreds of compounds, among them complex carbohydrates, a variety of organometallics, synthetic polyamides as well as complex alkaloids and other natural products, have been obtained using one of the afore mentioned ionization methods.  An ESI-APCI source is also available, but lacking an LC, use is limited to the sample infusion method.  A full-time spectrometrist performs all measurements and arranges work schedules. 

Finnigan Laser MAT 2000

A matrix-assisted laser desorption (MALDI) time of flight (TOF) mass spectrometer.  Ideally suited for analysis of proteins and higher molecular weight carbohydrates, albeit at low resolution only.

  1. Positive and negative ion detection