USING OF METAMORPH IN COMBINATION WITH SPIKE-2 FOR IN VIVO BRAIN IMAGING EXPERIMENTS

Metamorph

Acquire → Set Acquisition → Channel → Cascade 512B

Acquire → [New Window] → - show live

-binning

-set ROI

Metamorph

- stop live image

Port: 10 MHz of VSD or fast IOS

Acquire → Stream acquisition → enter number of frames (same as a at the Spike-2’s file)

--- set binning

--- set exposure time

Spike 2

Script → Run Script → Load Script → {C://data/macros/threspull_Multitrial.S2S

Or

C://data/macros/threspull_Multitrial_Shutter.S2S

(In case of using of shutter)}

--- OK

--- Sample Start

---- Sample Stop

---- New File → enter number of frames (for example, 2000)

---- OK

---- Sample Start

Spike-2

----Check baseline of the TTL at the Spike-2 (must be about 0 )

Metamorph

- Acquire → Stream acqusition

Spike-2

- check recording, duration of trial and stimulations at Spike-2

Metamorph

Acquire; check recorded file at the middle: light intensity should be around 30 000 – 60 000; if something wrong, go back to beginning

---Set sequential file name and directory; for example: My_rat01_, C:\\my_rats\

Spike-2

---- sample stop

---- New File → enter number of frames (for example, 2000)

---- OK

---- Sample Start

Metamorph

Journal → Loop → Loop a Journal → Select Journal: Loop_Recording.JNL

Convert files from STK to SPE:

--- start Metamorph

---Start file converter--- read stk2ste ---- start --- enter the pathway [C:\data\May32\]