Study Question Sets for Exam
2
Study Question Set 11
Cell Secretory Pathway - Golgi 1
Golgi Structure, Polarity, and Function
- Describe the experiment by which
George Palade identified the route taken by secretory proteins from their
site of synthesis to their site of secretion
- What is a "GFP tag"?
What information does a "GFP tag" give?
- How are GFP tags introduced
into a cell?
- How could GFP tags be used in an experiment to trace the movement of a protein from its site of synthesis to its final destination? Are there limitations on the type of information obtained? Explain.
- Describe the location,
structure and function of the CGN and the TGN in relation to the rest
of the components of the Golgi apparatus.
- What are "ER exit sites"?
- Describe the types of biochemical
modifications catalyzed by enzymes of the Golgi complex.
- Describe the different compartments of the secretory pathway responsible
for the synthesis, addition to protein, and maturation of N-linked oligosaccharide
chains of glycoproteins. Indicate the nature of the contribution(s) of
each compartment or subcompartment
- Explain briefly why there
are many different structures for N-linked oligosaccharide chains.
- Describe the role(s) of
the Golgi apparatus in the formation of proteoglycans.
- Why would cells synthesizing
large amounts of secretory polysaccharides have an elaborate Golgi complex
even if they have only moderate amounts of RER? Explain.
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