Study Question Sets for Exam 1
Study Question Set 3
Immunocytochemistry and Enzyme Cytochemistry

  1. How does one perform immunocytochemical localization of a protein?
  2. What features(s) allow one to do immunocytolocalization of more than one structure simultaneously?
  3. What is "Indirect Immunolcytochemistry"?
  4. How does one do immunocytolocalization with TEM?
  5. What kinds of enzymes are packaged into lysosomes? Relate them to lysosomal function.
  6. What is the Gomori reaction?
  7. Describe how one would use enzyme cytochemistry ( with the Gomori reaction) to identify lysosomes in cells with TEM. Be complete.
  8. What is the significance of the packaging of enzymes into specialized organelles such as lysosomes or peroxisomes in terms of function?
  9. What types of enzymes are packaged into peroxisomes?
  10. What are the roles of H2O2 and DAB in the technique for localizing peroxidase in discrete structures in cells?
  11. What properties make 3,3'-diaminobenzidine a good reductant for cytochemical demonstration of peroxidase activity?
  12. Describe how one would go about identifying peroxisomes in cells at the level of TEM using enzyme cytochemistry. Be complete.

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Last updated 1/21/2006
url:http://www.umsl.edu/~starling/


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