Wordlists for Exam 2
Lectures 15 & 16
RER Function
Synthesis on free polysomes
Soluble cytoplasmic proteins
Nuclear proteins
Peroxisomal proteins
Nuclear-encoded proteins for mitochondria, chloroplasts
Cell Secretory Pathway
Secretory proteins
Soluble and membrane proteins of lysosomes, endosome system, ER, Golgi
Integral proteins of plasma membrane
Peripheral proteins at extracellular surface of plasma membrane
Golgi apparatus = Golgi complex
cotranslational versus post-translational
Signal Sequence
Gunther Blobel - Nobel Prize 1999
Postmicrosomal fraction
in vitro translation
microsomes
Signal Recognition Particle (SRP)
6 protein subunits + 7S (300 base) RNA in eukaryotes
"hinge"
SRP Receptor
RER Translocator protein = translocon
Sec61 complex in eukaryotes: Sec61 alpha, Sec61 beta, Sec61 gamma
disulfide bond: -SH + -SH ---> -S--S-
PDI = protein disulfide isomerase
core oligosaccharide
dolichol
glycosyl transferase
oligosaccharyl transferase
disulfide isomerase
calnexin, calreticulin
N-glycanase
SecYEG in bacteria - Homologous to Sec61 complex of eukaryotes
sec A
BiP = "binding protein" - a hsp70-type molecular chaperone
"stop transfer sequence" = membrane anchor sequence
internal uncleaved signal sequence =
"start transfer sequence"
Also a membrane anchor sequence
hydrophobicity plot
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