Wordlists for Exam 1
Lectures 3 and 4
Visualizing Cells Parts 1 & 2

phase contrast microscopy

- addition of light waves in phase
- subtraction of light waves out of phase
interference contrast microscopy

Preparation of Tissue for Staining:

Fix
Dehydrate
Embed
Section
Stain

Aldehyded fixatives:

Glutaraldehyde and Formaldehyde
Fix proteins by reacting with free amino groups

Dehydration = replacement of water

Microtome

Resolving Power of Optical Systems   -   Limit of Resolution

electron dense, electron opaque
electron lucent
Pb(OH)₂, Pb-citrate
uranyl acetate (Ur₂O₂[C₂H₃O₂]₂)

Osmium tetroxide = OsO₄   (= "osmium")
 
Sequential fixation:

Fix with aldehydes
Post-fix with OsO₄

Ultramicrotome

TEM = Transmission Electron Microscopy/Micrograph
SEM = Scanning Electron Microscopy

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