Nelli N. Malysheva, Ph. D.

Current Address:

N.D.Zelinsky Institute of Organic chemistry,
Russian Academy of Sciences,
Leninsky pr. 47, 117901 Moscow, Russia
Tel +7(095)9383609

Fax +7(095)1355328

CURRENT POSITION

February 1968 - Junior scientist, Scientist, Senior scientist, Laboratory of Carbohydrates

Present - N.D.Zelinsky Institute of Organic Chemistry, Russian Academy of

Sciences, Leninsky pr. 47, 117901 Moscow, Russia.

Nature of Study: A. Synthesis of repeating oligosaccharide units of bacterial polysaccharides. B. Synthesis of oligosaccharides related to Rhizobia nodulation factors.

Responsibilities: Co-supervisor of two graduate students.

EDUCATION

November 1973 Ph. D Degree in Organic Chemistry, N. D. Zelinsky Institute of Organic Chemistry (IOC), Russian Academy of Sciences (RAS), 47 Leninsky Prospect, Moscow 117913, Russia.

Advisors: Professors Nikolai K. Kochetkov and Oleg S.Chizhov.

Thesis Title: "New derivatives of oligosaccharides for masspectrometrical determination of sequence".

June 1965 Diploma in Chemistry, Moscow State University, 1 Lenin Hills Moscow, Russia

AREAS OF EXPERTISE:

Chemical and chemo-enzymatic synthesis of the repeating units of natural polysaccharides with biological importance.
Synthesis of biologically important oligosaccharides related to Rhizobia nodulation factors
Bruker based NMR techniques (1D, APT, DEPT, COSY, TOCSY, HSQC, HMBC, HSQC-TOCSY, etc.), HPLC-NMR; UV; IR; Mass spectroscopy (MALDI, FAB, ES, GC-MS, HPLC-MS), polarimetry, circular dichroism.
Modern chromatography methods (GC, LC, HPLC, Dionex, flash, size-exclusion, gel, ion-exchange, thin layer, reverse phase, LLPC, etc.).

SELECTED PUBLICATIONS

A.I.Zinin, V.I.Torgov, V.N.Shibaev, A.S.Shashkov, N.N.Malysheva, and W.J.Broughton. Synthesis of lipooligosaccharides related to nodulation factors of Rhizobium sp. NGR 234. 1. Branched trisaccharides containing sulfated or acetylated residue of 2-O-methyl-L-fucose. Russian Chem.Bull., Int.Ed., 1998, 47, No3, 496-501.
A. V. Demchenko, N. N. Malysheva, N. K. Kochetkov. Influence of experimental conditions on proceeding of trityl-thiocyanate condensation reaction. Doklady Akademii Nauk, 1995, 341, 635-637.
E. M. Klimov, A. V. Demchenko, N. N. Malysheva. On interaction of acylated 2-deoxy-a-D-arabino-hexopyranosyl bromides with potassium thiocyanate. Doklady Akademii Nauk, 1993, 331, 53-55.
N. K. Kochetkov, E. M. Klimov, N. N. Malysheva, A. V. Demchenko. Stereospecific synthesis of 1,2-cis-glycosides of 2-amino sugars. Carbohydr. Res., 1993, 242, C7-C10.
E. M. Klimov, N. N. Malysheva, A. V. Demchenko, N. K. Kochetkov. Synthesis of 1,2-cis-linked regular heteropolysaccharide. Rhamnoglucan. Doklady Akademii Nauk, 1993, 330, 330-332.
E. M. Klimov, N. N. Malysheva, A. V. Demchenko, N. K. Kochetkov. Stereospecific synthesis of 1,2-cis-glycosides of 2-amino sugars. Doklady Akademii Nauk, 1992, 325, 297-301.
N. K. Kochetkov, E. M. Klimov, N. N. Malysheva, A. V. Demchenko. Stereospecific 1,2-cis-glycosylation: a modified thiocyanate method. Carbohydr. Res., 1992, 232, C1-C5.
N. K. Kochetkov, N. N. Malysheva, E. M. Klimov, A. V. Demchenko. Synthesis of polysaccharides with 1,2-cis-glycosidic linkages by trityl-thiocyanate polycondensation. Stereoregular a-(1--6)-D-glucan. Tetrahedron Lett., 1992, 33, 381-384.
N. K. Kochetkov, E. M. Klimov, N. N. Malysheva, A. V. Demchenko. A new modification of stereospecific 1,2-cis-glycosylation by thiocyanate method. Doklady Akademii Nauk, 1991, 321, 1203-1207.
N. K. Kochetkov, N. N. Malysheva, E. M. Klimov, A. V. Demchenko. Synthesis of 1,2-cis-linked polysaccharides via trityl-thiocyanate polycondensation. Doklady Akademii Nauk, 1991, 320, 634-637.
E. M. Klimov, A. V. Demchenko, N. N. Malysheva, N. K. Kochetkov. The synthesis of b-mannosides by glycosylation of a-mannosyl thiocyanates. Bioorg. Khim., 1991, 17, 1660-1664.
N. K. Kochetkov, N. N. Malysheva, A. V. Demchenko, et al.. Synthesis of 1,2-trans-glycosyl thiocyanates from 1,2-anhydro sugars. Bioorg. Khim., 1991, 17, 1655-1659.
N. K. Kochetkov, E. M. Klimov, N. N. Malysheva, A. V. Demchenko. A new stereospecific method for 1,2-cis-glycosylation. Carbohydr. Res., 1991, 212, 77-91.
N. K. Kochetkov, E. M. Klimov, N. N. Malysheva, A. V. Demchenko. A novel highly stereospecific method of 1,2-cis-glycosylation. Synthesis of a-D-glucosyl- and a-D-galactosyl-D-hexoses. Bioorg. Khim., 1990, 16, 701-710.
E. M. Klimov, N. N. Malysheva, A. V. Demchenko, et al. The effect of high pressure on the stereochemistry of glycosylation of sugars by 1,2-trans-acetates. Synthesis of gentioligosaccharides. Doklady Akademii Nauk, 1989, 309, 110-114.