Keith J. Stine
Professor
Stine graduated with special honors with a B.S. in Chemistry
from Fairleigh Dickinson University and received his Ph.D.
from Massachusetts Institute of Technology. He was a postdoctoral
fellow at UCLA and joined the UM-St. Louis Chemistry Department
in the Fall of 1990.
kstine@umsl.edu
Office: N204
Phone: (314) 516-5346
Fax: (314) 516-5342
Research Interests
Dr. Stine's research effort focuses on studies of monolayers and aggregates of lipids and surfactants, especially those of interest from the viewpoints of supramolecular chemistry and the study of biological membranes. Monolayers can serve as model systems providing insight into the physical properties of membranes and can be used to model molecular recognition processes occurring at membrane surfaces. Related systems of interest include micelles, liposomes, supported bilayers, and self-assembled monolayers. Monolayers of surface-active molecules at the air-water interface (Langmuir monolayers) are studied using fluorescence microscopy, Brewster angle microscopy, and surface pressure versus molecular area isotherm measurements. The transfer of these monolayers onto solid supports for examination by microscopy, spectroscopy, or electrochemistry is another area of interest. Self-assembled monolayers on gold surfaces are being examined using surface plasmon resonance and electrochemical methods. One of the main molecular features of interest has been the effect of chirality on the organization of these molecules. Applications of these systems can be found in the development of biosensors and in products for use in biotechnology applications.
Selected Publications
"Reverse Orthogonal Strategy for Oligosaccharide Synthesis", K. Fujikawa, N. V. Ganesh, Y. H. Tan, K. J Stine and A. V. Demchenko, Chem. Commun. 2011, 10602
“Characterization of Protein Immobilization on Nanoporous Gold using Atomic Force Microscopy and Scanning Electron Microscopy”, Y. H. Tan, J. R. Schallom, N. V. Ganesh, K. Fujikawa, A. V. Demchenko and K. J. Stine, Nanoscale, 2011, 3, 3395.
"Atomic Force Microscopy Characterization of Lipid/Protein Nanostructures Formed in Langmuir-Blodgett Films", Y. H. Tan and K. J. Stine, in Langmuir Monolayers in Thin Film Technology edited by J. A. Sherwin, Nova Science Publishers (Hauppage, New York), 2011 pp. 101-130,.
"Bioconjugation Reactions for Covalent Coupling of Proteins to Gold Surfaces", Y. H. Tan, B. Pandey, A. Sharma, J. Bhattarai, and K. J. Stine, Global Journal of Biochemistry, 2011, in press.
"Brewster Angle Microscopy", K. J. Stine, in Supramolecular Chemistry: From Molecules to Nanomaterials, Wiley-Interscience, Philip A. Gale and Jonathan W. Steed, Editors, 2011, in press.
"Nanoporous gold for enzyme immobilization"'. K. J Stine, K. Jefferson and O. V. Shulga, Methods in Molecular Biology, 2011, 679, 67.
“STICS: Surface-Tethered Iterative Carbohydrate Synthesis” Papapida Pornsuriyasak, Sneha Ranade, Aixiao Li, M. Cristina Parlato, Charles R. Sims, Olga V. Shulga, Keith J. Stine, and Alexei V. Demchenko, Chemical Communications 2009, 1834.
“Assessment of the Influence Factors on In Vitro Testing of Nasal Sprays Using Box-Behnken Experimental Design”, C. Guo, K. J. Stine, J. F. Kauffman, and W. H. Doub. European Journal of Pharmaceutical Sciences, 2008, 35, 417 .
"Comparison of the Interaction of Tomatine with Mixed Monolayers Containing Phospholipid, Egg Sphingomyelin, and Sterols", B. W. Walker, N. Manhanke, K. J. Stine, Biochimica et Biophysica Acta, 2008, 1778, 2244.
"Air-Water Interfacial Behavior of Amphiphilic Peptide Analogs of Synthetic Chloride Ion Transporters", E. K. Elliot, K. J. Stine, and G. W. Gokel, Journal of Membrane Science, 2008, 321, 43.
"Detection of free prostate specific antigen (fPSA) on a nanoporous gold platform", O. V. Shulga, D. Zhou, A. V. Demchenko and K. J. Stine, Analyst, 2008, 133, 319
"Dose Formulation and Analysis of Diapocynin", R.. Luchtefeld, R. Luo, Rensheng; K. J. Stine, M. L. Alt, P. A. Chernovitz and R. E. Smith, Journal of Agricultural and Food Chemistry, 2008, 56, 301
"Application of Glycosyl Thioimidates in Solid-Phase Oligosaccharide Synthesis", M. C. Parlato, M. N. Kamat, H. Wang, K. J. Stine and A. V. Demchenko, Journal of Organic Chemistry, 2008, 73, 1716.
"Monolayers of Triaroylbenzene Derivatives”, Mayuri K. Dighe, Frank J. Dover, Keith J. Stine, and F. Christopher Pigge, Thin Solid Films, 2008, 516, 3227.
"Preparation and Characterization of Porous Gold and its Application as a Platform for Immobilization of Acetylcholinesterase", O. V. Shulga, K. Jefferson, A. R. Khan, V. T. D'Souza, J. Liu, A. V. Demchenko, and K. J. Stine, Chemistry of Materials, 2007, 19, 3902.