Keith J. Stine
Professor
Stine graduated with special honors with a B.S. in Chemistry
from Fairleigh Dickinson University and received his Ph.D.
from Massachusetts Institute of Technology. He was a postdoctoral
fellow at UCLA and joined the UM-St. Louis Chemistry Department
in the Fall of 1990.
kstine@umsl.edu
Office: N204
Phone: (314) 516-5346
Fax: (314) 516-5342
Research Interests
Dr. Stine's research effort focuses on studies of monolayers and aggregates of lipids and surfactants, especially those of interest from the viewpoints of supramolecular chemistry and the study of biological membranes. Monolayers can serve as model systems providing insight into the physical properties of membranes and can be used to model molecular recognition processes occurring at membrane surfaces. Related systems of interest include micelles, liposomes, supported bilayers, and self-assembled monolayers. Monolayers of surface-active molecules at the air-water interface (Langmuir monolayers) are studied using fluorescence microscopy, Brewster angle microscopy, and surface pressure versus molecular area isotherm measurements. The transfer of these monolayers onto solid supports for examination by microscopy, spectroscopy, or electrochemistry is another area of interest. Self-assembled monolayers on gold surfaces are being examined using surface plasmon resonance and electrochemical methods. One of the main molecular features of interest has been the effect of chirality on the organization of these molecules. Applications of these systems can be found in the development of biosensors and in products for use in biotechnology applications.
Selected Publications
“STICS: Surface-Tethered Iterative Carbohydrate Synthesis” Papapida Pornsuriyasak, Sneha Ranade, Aixiao Li, M. Cristina Parlato, Charles R. Sims, Olga V. Shulga, Keith J. Stine, and Alexei V. Demchenko, Chemical Communications 2009, 1834.
“Assessment of the Influence Factors on In Vitro Testing of Nasal Sprays Using Box-Behnken Experimental Design”, C. Guo, K. J. Stine, J. F. Kauffman, and W. H. Doub. European Journal of Pharmaceutical Sciences, 2008, 35, 417 .
"Comparison of the Interaction of Tomatine with Mixed Monolayers Containing Phospholipid, Egg Sphingomyelin, and Sterols", B. W. Walker, N. Manhanke, K. J. Stine, Biochimica et Biophysica Acta, 2008, 1778, 2244.
"Air-Water Interfacial Behavior of Amphiphilic Peptide Analogs of Synthetic Chloride Ion Transporters", E. K. Elliot, K. J. Stine, and G. W. Gokel, Journal of Membrane Science, 2008, 321, 43.
"Detection of free prostate specific antigen (fPSA) on a nanoporous gold platform", O. V. Shulga, D. Zhou, A. V. Demchenko and K. J. Stine, Analyst, 2008, 133, 319
"Dose Formulation and Analysis of Diapocynin", R.. Luchtefeld, R. Luo, Rensheng; K. J. Stine, M. L. Alt, P. A. Chernovitz and R. E. Smith, Journal of Agricultural and Food Chemistry, 2008, 56, 301
"Application of Glycosyl Thioimidates in Solid-Phase Oligosaccharide Synthesis", M. C. Parlato, M. N. Kamat, H. Wang, K. J. Stine and A. V. Demchenko, Journal of Organic Chemistry, 2008, 73, 1716.
"Monolayers of Triaroylbenzene Derivatives”, Mayuri K. Dighe, Frank J. Dover, Keith J. Stine, and F. Christopher Pigge, Thin Solid Films, 2008, 516, 3227.
"Preparation and Characterization of Porous Gold and its Application as a Platform for Immobilization of Acetylcholinesterase", O. V. Shulga, K. Jefferson, A. R. Khan, V. T. D'Souza, J. Liu, A. V. Demchenko, and K. J. Stine, Chemistry of Materials, 2007, 19, 3902.
"Interaction of the Glycoalkaloid Tomatine with DMPC and Sterol Monolayers Studied by Surface Pressure Measurements and Brewster Angle Microscopy", K. J. Stine, R. K. Hercules, J. D. Duff, and B. W. Walker, Journal of Physical Chemistry B, 2006, 110, 22220.
"Appearance and Disappearance of Dendritic and Chiral Patterns in Domains of Langmuir Monolayers Observed with Brewster Angle Microscopy", F. V. Hoffmann, K. J. Stine, and H. Hühnerfuss, Journal of Physical Chemistry B. 2005, 109, 240 .
"Simple Lattice Simulation of Chiral Discrimination in Monolayers", L. Mao, H. H. Harris, K. J. Stine. Journal of Chemical Information and Computer Sciences, 2002, 42, 1179.
"Chirality in Monolayers", K. J. Stine, in Encyclopedia of Surface and Colloid Science, (A. Hubbard, Ed.) Marcel Dekker: New York, 2002, pp. 1017.
"Chirality In Membranes:
First Evidence That Enantioselective Interactions Between Cholesterol and
Cell Membrane Lipids Can Be A Determinant of Membrane Physical Properties", S.
Lalitha, A. S. Kumar, K. J. Stine and D. F. Covey, Journal of Supramolecular
Chemistry 2001, 1, 53.
"Langmuir Monolayers-Fundamentals and Relevance to Nanotechnology", K. J. Stine
and B. G. Moore, Chapter 3 of Nano-Surface Chemistry, (M. Rosoff, Ed.), Marcel
Dekker: New York, 2001, pp. 59-140.
"Enantiospecificity of Sterol-Lipid Interactions: First Evidence
that the Absolute Configuration of Cholesterol Affects the Physical Properties
of Cholesterol- Sphingomyelin Membranes", S. Laalithaa, A. S. Kumar,
K. J. Stine, and D. F. Covey, Chemical Communications, 2001,
1192-1193.
"Fluorescence Microscopy For Studying Biological Model Systems - Phospholipid
Monolayers and Chiral Discrimination Effects ", K. J. Stine, Chapter
22 of Physical Chemistry of Biological Interfaces, (W. Norde and A. Baszkin,
Eds.), Marcel Dekker: New York, 2000, pp. 749-768
"Structure and Electrochemical Behavior of a Flavin Sulfide Monolayer on
Gold", K. J. Stine, D. M. Andrauskas, A. R. Khan, P. Forgo, V. T. D'Souza,
R. M. Friedman, and J. Liu, Journal of Electroanalytical Chemistry, 1999, 472,
147.
Complete list
of publications