High Field NMR Facility

The University of Missouri-St Louis High Resolution NMR Facility is located in the Department of Chemistry and Biochemistryon the second floor of Benton Hall, room B210 and houses three NMR spectrometers: a Bruker ARX-500 , a Bruker Avance 300 and a Varian Unity Plus 300 with an Oxford wide-bore magnet. While these instruments are primarily for the use of the faculty, postdoctoral, and students in the Department of Chemistry and Biochemistry, other users (corporate or other university, organization) are welcome. The facility staff will provide NMR services as needed. For more information please contact to Rensheng Luo, at (314)516-5330.

Bruker ARX 500 MHz NMR

The Bruker ARX-500 spectrometer was installed in 1993 and upgraded in 2001. This spectrometer is operated by a Silicon Graphics INDY R5000 workstation. It has two probes: a 5-mm broadband and a 5 mm inverse gradient broadband, each of which is capable of variable temperature experiments (-150 to + 200 ºC for liquids). The Bruker ARX-500 spectrometer is a research-oriented instrument. It is equipped with dual radiofrequency channels and used for molecules requiring better peak resolution, (complex) structure elucidation and variable temperature analyses on a wide range of organic, organometallic, inorganic, and biochemical systems, as well as natural products and host-guest systems. Experiments that are typically performed include 2D COSY, NOESY, ROESY, TOCSY, HSQC, HMQC, HMBC and selective excitation. Users can be trained to operate the ARX500 and must demonstrate proficiency if operating the instrument by users.

Oxford magnet (54 mm bore)
SGI R5000 workstation
BSMS magnetic control system
5mm switchable inverse broadband tunable for: ¹H-¹⁹F/¹⁵N-³¹P with z-gradient and boron-free glass insert
5 mm switchable broadband probe tunable for: ¹H -¹⁹F/¹⁵N -³¹P
Variable temperature capability (-150º C to +200º C)

Bruker Avance 300MHz NMR

In 2001, a Varian XL-300 console and electronics were upgraded to a Bruker Avance 300 spectrometer. The Avance 300 spectrometer is the departmental workhorse instrument. It is currently equipped with a four-nucleus probe (¹H, ¹³C, ¹⁹F, and ³¹P) with z-gradients and variable temperature capability. An additional 5 mm switchable broadband probe tunable for ¹H-¹⁹F/¹⁵N -³¹P is also available. This instrument is used primarily for routine walk-on (daytime between 8:00 am to 8:00 pm) use for monitoring reactions, and checking the purity of samples, but it is also used for longer run experiments such as 1D ¹³C and routine 2D experiments: COSY, HMQC, HSQC, and HMBC in the evening (after 8:00 pm) and weekends.

Oxford magnet (54 mm bore)
PC/NT Pentium III 750MHz computer
BSMS magnetic control system
5mm four-nucleus probe observable for ¹H, ¹³C, ¹⁹F, and ³¹P with z-gradient
5mm switchable broadband probe (boron-free), tunable for ¹H -¹⁹F/¹⁵N -³¹P
Variable temperature capability (-150º C to +200º C)

Varian Unity Plus 300 MHz NMR

The Varian Unity Plus 300 was installed in early 1995. It is equipped with a wide bore Oxford superconducting magnet to accommodate probes for running solid state NMR experiments. It has two radiofrequency channels and is capable of broadband detection. This instrument is used primarily for detection of heteronuclei such as ¹¹B, ¹³C, ³¹P, ¹¹⁹Sn, and ¹⁹⁵Pt, experiments that require long detection time, including dilute samples, and for two-dimensional experiments. It has four probes: two 5-mm switchable broadband probes with boron-free glass insert, a 5-mm switchable inverse broadband probe, and a 7-mm magic angle probe for CP-MAS experiments.

Oxford magnet (54 mm bore)
PC/NT Pentium III 750MHz computer
BSMS magnetic control system
5mm four-nucleus probe observable for ¹H, ¹³C, ¹⁹F, and ³¹P with z-gradient
5mm switchable broadband probe (boron-free), tunable for ¹H -¹⁹F/¹⁵N -³¹P
Variable temperature capability (-150º C to +200º C)

All questions regarding the use of the the high resolution NMR facility should be directed to Dr. Rensheng Luo or visit his personal website