University of Missouri-St Louis High Resolution NMR Facility is located in the Department of Chemistry and Biochemistry on the second floor of Benton Hall, room B209, room B207, and houses four NMR spectrometers: an Agilent DD2-600, a Bruker ARX-500, a Bruker Avance 300 and a Varian Unity Plus 300 with an Oxford wide-bore magnet. While these instruments are primarily for the use of the faculty, postdoctoral, and students in the Department of Chemistry and Biochemistry, other users (corporate or other university, organization) are welcome. The facility staff will provide NMR services as needed. For more information please contact Dr. Luo, at (314)516-5330.

Agilent DD2 600 MHz NMR

We acknowledge support from NSF MRI-R2 grant (0959360). An Agilent DD2 600 MHz NMR spectrometer was purchased and installed in Benton Hall B207 in early 2012. This spectrometer is operated by Linux PC with VnmrJ 3.2 software. Two probes are available: a 5-mm three channel inverse gradient broadband and a 5-mm gradient broadband, each of which is capable of variable temperature experiments (-80 to +130oC). This spectrometer is a research-oriented instrument and primarily used to investigate the structures and dynamics of macromolecules and complex molecular systems as well as implement new NMR experiments for users at different areas.

  Instrument specifications:
  • The Agilent preminumCOMPACT magnet (54mm bore)
  • Dell PC with Red Hat Linux
  • 1H-19F/15N-31P, 15N/13C 5mm PFG triple OneNMR probe
  • 1H-19F/15N-31P 5mm PFG autoX indirect detection probe
  • ProTune accessory
  • Variable temperature capability (-80oC to +130oC)
NMR  

 Bruker ARX 500 MHz NMR

The Bruker ARX-500 spectrometer was installed in 1993 and upgraded in 2001. This spectrometer is operated by a Silicon Graphics INDY R5000 workstation. Two probes are available: a 5-mm broadband and a 5 mm inverse gradient broadband, each of which is capable of variable temperature experiments (150 to + 200oC). The instrument is equipped with dual radiofrequency channels and used for molecules requiring better peak resolution, (complex) structure elucidation and variable temperature analyses on a wide range of organic, organometallic, inorganic, and biochemical systems, as well as natural products and host-guest systems. Experiments that are typically performed include 2D COSY, NOESY, ROESY, TOCSY, HSQC, HMQC, HMBC and selective excitation. Users can be trained to operate the ARX 500 and must demonstrate proficiency on the operation of the instrument.

Instrument specifications:

  • Oxford magnet (54 mm bore)
  • SGI R5000 workstation
  • BSMS magnetic control system
  • 5mm switchable inverse broadband tunable for: 1H-19F/15N-31P with z-gradient and boron-free glass insert
  • 5 mm switchable broadband probe tunable for: 1H -19F/15N -31P
  • Variable temperature capability (-150oC to +200oC)

Bruker Avance 300 MHz NMR

In 2001, a Varian XL-300 console and electronics were upgraded to a Bruker Avance 300 spectrometer. The Avance 300 spectrometer is the departmental workhorse instrument. It is currently equipped with a four-nucleus probe (1H, 13C, 19F, and 31P) with z-gradients and variable temperature capability. An additional 5 mm switchable broadband probe tunable for 1H-19F/15N -31P is also available. This instrument is used primarily for routine walk-on (daytime between 8:00 am to 8:00 pm) use for monitoring reactions, and checking the purity of samples, but it is also used for longer run experiments such as 1D 13C and routine 2D experiments: COSY, HMQC, HSQC, and HMBC in the evening (after 8:00 pm) and weekends.

Instrument specifications:

  • Oxford magnet (54 mm bore)
  • PC/NT Pentium III 750MHz computer
  • BSMS magnetic control system
  • 5mm four-nucleus probe observable for 1H, 13C, 19F, and 31P with z-gradient
  • 5mm switchable broadband probe (boron-free), tunable for 1H -19F/15N -31P

Varian Unity Plus 300 MHz NMR

The Varian Unity Plus 300 was installed in early 1995. It is equipped with a wide bore Oxford superconducting magnet to accommodate probes for running solid state heteronuclear NMR experiments. It has two radiofrequency channels and is capable of broadband detection. This instrument is used primarily for detection of heteronuclei such as 11B, 13C, 31P, 119Sn, and 195Pt, experiments that require long detection time, including dilute samples, and for two-dimensional experiments. Four probes are availalbe: two 5-mm switchable broadband probes with boron-free glass insert, a 5-mm switchable inverse broadband probe, and a 7-mm magic angle probe for CP-MAS experiments.

Instrument specifications:

  • Oxford magnet (54 mm bore)
  • PC/NT Pentium III 750MHz computer
  • BSMS magnetic control system
  • 5mm four-nucleus probe observable for 1H, 13C, 19F, and 31P with z-gradient
  • 5mm switchable broadband probe (boron-free), tunable for 1H -19F/15N -31P

All questions regarding the use of the high resolution NMR facility should be directed to Rensheng Luo or by visiting our nmr website